Protocol for muscle fiber type and cross-sectional area analysis in cryosections of whole lower mouse hindlimbs

Edmund Battey*, Matthieu Dos Santos, Dipsikha Biswas, Pascal Maire, Kei Sakamoto*

*Corresponding author for this work

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Abstract

We outline a protocol to visualize all mouse lower hindlimb skeletal muscles simultaneously. We describe procedures for orientating the whole lower hindlimb in gum tragacanth prior to freezing, simplifying the proceeding experimental steps, and enhancing the comprehensiveness of characterizations. We then detail steps for quantifying muscle fiber size and fiber type characteristics in a single cryosection using histochemistry and immunofluorescence. This protocol can be applied to histological and (immuno)histochemical evaluations such as muscle regeneration, fibrosis, enzymatic activity, and glycogen content.

Original languageEnglish
Article number103424
JournalSTAR Protocols
Volume5
Issue number4
Number of pages14
ISSN2666-1667
DOIs
Publication statusPublished - 2024

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Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.

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