Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise

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Abstract

The tissue-specific release and uptake of metabolites in response to exercise is incompletely understood. Here, we detail a protocol to assess arteriovenous differences across the liver and hindlimb muscles in response to treadmill exercise in mice. We describe steps for the treadmill running of mice and the region-specific sampling of blood from the liver and hindlimb. This procedure is particularly relevant for the study of tissue-specific metabolism in response to exercise. For complete details on the use and execution of this protocol, please refer to Sato et al. (2022). 1.

Original languageEnglish
Article number101985
JournalSTAR Protocols
Volume4
Issue number1
Number of pages11
ISSN2666-1667
DOIs
Publication statusPublished - 2023

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Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

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