Quantification of protein modification by oxidants

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Abstract

Proteins are major targets for oxidative damage due to their abundance and rapid rates of reaction with a wide range of radicals and excited state species, such as singlet oxygen. Exposure of proteins to these oxidants results in loss of the parent amino acid residue, formation of unstable intermediates, and the generation of stable products. Each of these events can be used, to a greater or lesser extent, to quantify damage to proteins. In this review the advantages and disadvantages of a number of these approaches are discussed, with an emphasis on methods that yield absolute quantitative data on the extent of protein modification. Detailed methods sheets are provided for many of these techniques.

Original languageEnglish
JournalFree Radical Biology & Medicine
Volume46
Issue number8
Pages (from-to)965-88
Number of pages24
ISSN0891-5849
DOIs
Publication statusPublished - 15 Apr 2009
Externally publishedYes

Keywords

  • Animals
  • Biochemistry
  • Biological Markers
  • Chromatography, High Pressure Liquid
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Mass Spectrometry
  • Oxidants
  • Oxidation-Reduction
  • Oxidative Stress
  • Protein Processing, Post-Translational
  • Proteins
  • Reactive Oxygen Species
  • Spin Trapping
  • Statistics as Topic

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