Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland

Martin Fredensborg Rath; Morten Møller

doi:10.1007/978-1-0716-2593-4_10

Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland

Martin Fredensborg Rath^*, Morten Møller

^*Corresponding author for this work

Molecular Neuroscience

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

4 Citations (Scopus)

Abstract

Radiochemical in situ hybridization enables detection of gene expression in small areas of the brain, such as the developing pineal gland in rodents. The method combines determination of spatial and temporal gene expression profiles with semiquantitative analyses. We here describe the procedure of radiochemical in situ hybridization on the developing rat pineal gland ranging from preparation of fetal tissue for in situ hybridization to principles of quantification.

Original language	English
Title of host publication	Methods in Molecular Biology
Number of pages	10
Publisher	Humana Press
Publication date	2022
Pages	75-84
DOIs	https://doi.org/10.1007/978-1-0716-2593-4_10
Publication status	Published - 2022

Series	Methods in Molecular Biology
Volume	2550
ISSN	1064-3745

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Brain development
In situ hybridization
Pineal gland
Radioactive
Rodent

Access to Document

10.1007/978-1-0716-2593-4_10

Cite this

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abstract = "Radiochemical in situ hybridization enables detection of gene expression in small areas of the brain, such as the developing pineal gland in rodents. The method combines determination of spatial and temporal gene expression profiles with semiquantitative analyses. We here describe the procedure of radiochemical in situ hybridization on the developing rat pineal gland ranging from preparation of fetal tissue for in situ hybridization to principles of quantification.",

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