Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth

Anja Lindis Dahl; Martin Barfred Friis; Hanne Wiese Hallberg; Gitte Qvist Kristiansen; Barbara Juliane Holzknecht

doi:10.1016/j.diagmicrobio.2024.116233

Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth

Anja Lindis Dahl^*, Martin Barfred Friis, Hanne Wiese Hallberg, Gitte Qvist Kristiansen, Barbara Juliane Holzknecht

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Diagnostic accuracy of laboratory-developed PCR after overnight enrichment for the detection of vanB vancomycin-resistant enterococci was evaluated on 537 rectal swabs. Defining Ct-values of 27-34 (40 samples, 7 % inconclusive), we found an excellent sensitivity of 98,3 % and specificity of 99,7 % for the remaining 497 samples.

Original language	English
Article number	116233
Journal	Diagnostic Microbiology and Infectious Disease
Volume	109
Issue number	2
Number of pages	3
ISSN	0732-8893
DOIs	https://doi.org/10.1016/j.diagmicrobio.2024.116233
Publication status	Published - 2024

Bibliographical note

Publisher Copyright:
© 2024 The Authors

Keywords

Carriage
Enrichment
vanB
Vancomycin-resistant enterococci
VRE

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10.1016/j.diagmicrobio.2024.116233Licence: CC BY

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Cite this

Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth. / Dahl, Anja Lindis; Friis, Martin Barfred; Hallberg, Hanne Wiese; Kristiansen, Gitte Qvist; Holzknecht, Barbara Juliane.

In: Diagnostic Microbiology and Infectious Disease, Vol. 109, No. 2, 116233, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "Diagnostic accuracy of laboratory-developed PCR after overnight enrichment for the detection of vanB vancomycin-resistant enterococci was evaluated on 537 rectal swabs. Defining Ct-values of 27-34 (40 samples, 7 % inconclusive), we found an excellent sensitivity of 98,3 % and specificity of 99,7 % for the remaining 497 samples.",

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