Reconstruction of Optical Thickness from Hoffman Modulation Contrast Images

Niels Holm Olsen, Jon Sporring, Mads Nielsen, Christina Hnida, Søren Ziebe

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

2 Citations (Scopus)

Abstract

Hoffman microscopy imaging systems are part of numerous fertility clinics world-wide. We discuss the physics of the Hoffman imaging system from optical thickness to image intensity, implement a simple, yet fast, reconstruction algorithm using Fast Fourier Transformation and discuss the usability of the method on a number of cells from a human embryo. Novelty is identifying the non-linearity of a typical Hoffman imaging system, and the application of Fourier Transformation to reconstruct the optical thickness.
Original languageEnglish
Title of host publicationImage Analysis : 13th Scandinavian Conference, SCIA 2003 Halmstad, Sweden, June 29 – July 2, 2003 Proceedings
PublisherSpringer
Publication date2003
Pages445-454
ISBN (Print)978-3-540-40601-3
DOIs
Publication statusPublished - 2003
Event13th Scandinavian Conference in Image Analysis - SCIA - Halmstad, Sweden
Duration: 29 Nov 2010 → …
Conference number: 13

Conference

Conference13th Scandinavian Conference in Image Analysis - SCIA
Number13
Country/TerritorySweden
CityHalmstad
Period29/11/2010 → …
SeriesLecture notes in computer science
Volume2749/2003
ISSN0302-9743

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