Abstract
Background: Recovery and functional differentiation of T-cell subsets are central for the development of immune function and complications after allogeneic hematopoietic stem cell transplantation (HSCT), but little is known about the cellular respiration and factors influencing T-cell metabolic fitness during immune maturation after HSCT. Method: We included 20 HSCT patients and analysed mitochondrial oxidative phosphorylation and mitochondrial fitness in peripheral blood mononuclear cell samples collected at days +90 and +180 after HSCT. Results: Phenotypic analysis revealed lower overall T-cell counts, lower CD4+/CD8+ ratio and a skewed distribution of early T-cell subsets at day +90, gradually recovering by day +180. Although ATP turnover in HSCT patients was similar to healthy controls, the spare respiratory capacity (SRC) of T cells, reflecting the available energy reserve, was significantly reduced at day +90 and +180 compared to healthy controls. This reduction in SRC was not correlated with the occurrence of acute graft-versus-host disease (aGVHD), the intensity of conditioning regimens and markers of T-cell exhaustion. Conclusion: We found significantly depressed SRC until six months post-HSCT, but we were not able to identify transplant-related risk factors or associations with the clinical outcome.
Original language | English |
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Article number | 1327977 |
Journal | Frontiers in Immunology |
Volume | 14 |
ISSN | 1664-3224 |
DOIs | |
Publication status | Published - 2024 |
Bibliographical note
Publisher Copyright:Copyright © 2024 Mølgaard, Kielsen, Ifversen, Met, Svane and Müller.
Keywords
- acute graft-versus-host disease
- hematopoietic stem cell transplantation
- mitochondrial fitness
- real-time metabolism
- spare respiratory capacity
- T cells