Reliability and variation in mitochondrial respiration in human adipose tissue

Ronni Eg Sahl; Eva Frederikke Høy Helms; Malte Schmücker; Mathias Flensted-Jensen; Arthur Ingersen; Thomas Morville; Flemming Dela; Jørn Wulff Helge; Steen Larsen

doi:10.1080/21623945.2021.1991617

Reliability and variation in mitochondrial respiration in human adipose tissue

Ronni Eg Sahl^*, Eva Frederikke Høy Helms, Malte Schmücker, Mathias Flensted-Jensen, Arthur Ingersen, Thomas Morville, Flemming Dela, Jørn Wulff Helge, Steen Larsen

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

3 Citations (Scopus)

31 Downloads (Pure)

Abstract

Adipose tissue mitochondrial function is gaining increasing interest since it is a good marker of overall health. Methodological challenges and variability in assessing mitochondrial respiration in fresh adipose tissue with high-resolution respirometry are unknown and should be explored. Mitochondrial respiratory capacity (MRC) in human adipose tissue declines in a gradual manner when analyses are postponed 3 h and 24 h, with a statistically significant decline 24 h after obtaining the biopsy. This decline in MRC is associated with a reduced integrity of the outer mitochondrial membrane at both time points. This study suggests that the optimal amount of tissue to be used is 20 mg and that different technicians handling the biopsy do not affect MRC.

Original language	English
Journal	Adipocytes
Volume	10
Issue number	1
Pages (from-to)	605-611
Number of pages	7
ISSN	1550-2082
DOIs	https://doi.org/10.1080/21623945.2021.1991617
Publication status	Published - 2021

Bibliographical note

Publisher Copyright:
© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Keywords

Adipose tissue
coefficient of variation
high resolution respirometry
human
mitochondria

Access to Document

10.1080/21623945.2021.1991617Licence: CC BY

FulltextFinal published version, 1.32 MBLicence: CC BY

Cite this

Reliability and variation in mitochondrial respiration in human adipose tissue. / Sahl, Ronni Eg; Høy Helms, Eva Frederikke; Schmücker, Malte ; Flensted-Jensen, Mathias ; Ingersen, Arthur; Morville, Thomas; Dela, Flemming ; Helge, Jørn Wulff; Larsen, Steen.

In: Adipocytes, Vol. 10, No. 1, 2021, p. 605-611.

Research output: Contribution to journal › Journal article › Research › peer-review

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AU - Ingersen, Arthur

AU - Morville, Thomas

AU - Dela, Flemming

AU - Helge, Jørn Wulff

AU - Larsen, Steen

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