@inbook{5733ee00e51242ca8ec6f1e1093617db,
title = "Replication Labeling Methods for Super-Resolution Imaging of Chromosome Territories and Chromatin Domains",
abstract = "Continuing progress in super-resolution microscopy enables the study of sub-chromosomal chromatin organization in single cells with unprecedented detail. Here we describe refined methods for pulse-chase replication labeling of individual chromosome territories (CTs) and replication domain units in mammalian cell nuclei, with specific focus on their application to three-dimensional structured illumination microscopy (3D-SIM). We provide detailed protocols for highly efficient electroporation-based delivery or scratch loading of cell-impermeable fluorescent nucleotides for live-cell studies. Furthermore, we describe the application of (2'S)-2'-deoxy-2'-fluoro-5-ethynyluridine (F-ara-EdU) and 5-vinyl-2'-deoxyuridine (VdU) for the in situ detection of segregated chromosome territories and sister chromatids with minimized cytotoxic side effects.",
keywords = "Animals, Cell Nucleus, Chromatids, Chromatin/genetics, Mammals, Microscopy/methods",
author = "Ezequiel Miron and Joseph Windo and Fena Ochs and Lothar Schermelleh",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2221-6_9",
language = "English",
isbn = "978-1-0716-2223-0",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "111--128",
editor = "Leake, {Mark C.}",
booktitle = "Chromosome Architecture",
address = "Switzerland",
edition = "2",
}