Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer

Marco van de Weert; Riemke van Dijkhuizen-Radersma; Jeroen M Bezemer; Wim E Hennink; Daan J A Crommelin

Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer

Marco van de Weert, Riemke van Dijkhuizen-Radersma, Jeroen M Bezemer, Wim E Hennink, Daan J A Crommelin

Research output: Contribution to journal › Journal article › Research › peer-review

15 Citations (Scopus)

Abstract

Lysozyme-loaded poly(ethylene glycol terephthalate)-poly(butylene terephthalate) (PEGT/PBT) films were prepared using a water-in-oil emulsification solvent evaporation method. Infrared spectroscopic analysis of the dried films indicated the presence of non-covalent lysozyme aggregates in the polymer matrix. The use of methanol to enhance the drying rate of the films increased the relative amount of aggregates. Surprisingly, quantitative in-vitro release of fully active, non-aggregated lysozyme was observed, indicating that lysozyme forms reversible aggregates during encapsulation in PEGT/PBT films.

Original language	English
Journal	European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
Volume	54
Issue number	1
Pages (from-to)	89-93
Number of pages	5
Publication status	Published - Jul 2002
Externally published	Yes

Cite this

Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer. / van de Weert, Marco; van Dijkhuizen-Radersma, Riemke; Bezemer, Jeroen M; Hennink, Wim E; Crommelin, Daan J A.

In: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, Vol. 54, No. 1, 07.2002, p. 89-93.

Research output: Contribution to journal › Journal article › Research › peer-review

van de Weert, Marco ; van Dijkhuizen-Radersma, Riemke ; Bezemer, Jeroen M ; Hennink, Wim E ; Crommelin, Daan J A. / Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer. In: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V. 2002 ; Vol. 54, No. 1. pp. 89-93.

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abstract = "Lysozyme-loaded poly(ethylene glycol terephthalate)-poly(butylene terephthalate) (PEGT/PBT) films were prepared using a water-in-oil emulsification solvent evaporation method. Infrared spectroscopic analysis of the dried films indicated the presence of non-covalent lysozyme aggregates in the polymer matrix. The use of methanol to enhance the drying rate of the films increased the relative amount of aggregates. Surprisingly, quantitative in-vitro release of fully active, non-aggregated lysozyme was observed, indicating that lysozyme forms reversible aggregates during encapsulation in PEGT/PBT films.",

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AU - van de Weert, Marco

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AU - Hennink, Wim E

AU - Crommelin, Daan J A

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N2 - Lysozyme-loaded poly(ethylene glycol terephthalate)-poly(butylene terephthalate) (PEGT/PBT) films were prepared using a water-in-oil emulsification solvent evaporation method. Infrared spectroscopic analysis of the dried films indicated the presence of non-covalent lysozyme aggregates in the polymer matrix. The use of methanol to enhance the drying rate of the films increased the relative amount of aggregates. Surprisingly, quantitative in-vitro release of fully active, non-aggregated lysozyme was observed, indicating that lysozyme forms reversible aggregates during encapsulation in PEGT/PBT films.

AB - Lysozyme-loaded poly(ethylene glycol terephthalate)-poly(butylene terephthalate) (PEGT/PBT) films were prepared using a water-in-oil emulsification solvent evaporation method. Infrared spectroscopic analysis of the dried films indicated the presence of non-covalent lysozyme aggregates in the polymer matrix. The use of methanol to enhance the drying rate of the films increased the relative amount of aggregates. Surprisingly, quantitative in-vitro release of fully active, non-aggregated lysozyme was observed, indicating that lysozyme forms reversible aggregates during encapsulation in PEGT/PBT films.

M3 - Journal article

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JO - European Journal of Pharmaceutics and Biopharmaceutics

JF - European Journal of Pharmaceutics and Biopharmaceutics

SN - 0939-6411

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