Sequence selective double strand DNA Cleavage by Peptide Nucleic Acid (PNA) targeting using Nuclease S1

Vadim Demidov; Maxim D. Frank-Kamenetskii; M. Egholm; Ole Buchardt; Peter E. Nielsen

doi:10.1093/nar/21.9.2103

Sequence selective double strand DNA Cleavage by Peptide Nucleic Acid (PNA) targeting using Nuclease S1

Vadim Demidov
, Maxim D. Frank-Kamenetskii
, M. Egholm
, Ole Buchardt
, Peter E. Nielsen

Transcription, RNA, and Gene Medicine Program

Research output: Contribution to journal › Journal article › Research

Abstract

A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting Is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete cleavage can be obtalned. The efficiency of this cleavage is more than 10 fold enhanced when a tandem PNA site Is targeted, and additionally enhanced If this site Is In trans rather than in cis orientation. Thus In effect, the PNA targeting makes the single strand specific nuclease S1 behave like a pseudo restriction endonuclease.

Original language	English
Journal	Nucleic Acids Research
Volume	21
Issue number	9
Pages (from-to)	2103-2107
ISSN	0305-1048
DOIs	https://doi.org/10.1093/nar/21.9.2103
Publication status	Published - 1993

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@article{4513a81074cf11dbbee902004c4f4f50,

title = "Sequence selective double strand DNA Cleavage by Peptide Nucleic Acid (PNA) targeting using Nuclease S1",

abstract = "A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting Is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete cleavage can be obtalned. The efficiency of this cleavage is more than 10 fold enhanced when a tandem PNA site Is targeted, and additionally enhanced If this site Is In trans rather than in cis orientation. Thus In effect, the PNA targeting makes the single strand specific nuclease S1 behave like a pseudo restriction endonuclease.",

author = "Vadim Demidov and Frank-Kamenetskii, \{Maxim D.\} and M. Egholm and Ole Buchardt and Nielsen, \{Peter E.\}",

year = "1993",

doi = "10.1093/nar/21.9.2103",

language = "English",

volume = "21",

pages = "2103--2107",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press",

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}

TY - JOUR

T1 - Sequence selective double strand DNA Cleavage by Peptide Nucleic Acid (PNA) targeting using Nuclease S1

AU - Demidov, Vadim

AU - Frank-Kamenetskii, Maxim D.

AU - Egholm, M.

AU - Buchardt, Ole

AU - Nielsen, Peter E.

PY - 1993

Y1 - 1993

N2 - A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting Is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete cleavage can be obtalned. The efficiency of this cleavage is more than 10 fold enhanced when a tandem PNA site Is targeted, and additionally enhanced If this site Is In trans rather than in cis orientation. Thus In effect, the PNA targeting makes the single strand specific nuclease S1 behave like a pseudo restriction endonuclease.

AB - A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting Is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete cleavage can be obtalned. The efficiency of this cleavage is more than 10 fold enhanced when a tandem PNA site Is targeted, and additionally enhanced If this site Is In trans rather than in cis orientation. Thus In effect, the PNA targeting makes the single strand specific nuclease S1 behave like a pseudo restriction endonuclease.

U2 - 10.1093/nar/21.9.2103

DO - 10.1093/nar/21.9.2103

M3 - Journal article

SN - 0305-1048

VL - 21

SP - 2103

EP - 2107

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 9

ER -