Soil organic matter properties drive microbial enzyme activities and greenhouse gas fluxes along an elevational gradient

Xingguo Han*, Anna Doménech-Pascual, Joan Pere Casas-Ruiz, Jonathan Donhauser, Karen Jordaan, Jean Baptiste Ramond, Anders Priemé, Anna M. Romaní, Aline Frossard

*Corresponding author for this work

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Abstract

Mountain ecosystems, contributing substantially to the global carbon (C) and nitrogen (N) biogeochemical cycles, are heavily impacted by global changes. Although soil respiration and microbial activities have been extensively studied at different elevation, little is known on the relationships between environmental drivers, microbial functions, and greenhouse gas fluxes (GHGs; carbon dioxide [CO2], methane [CH4] and nitrous oxide [N2O]) in soils of different elevation. Here, we measured how in situ GHG fluxes were linked to soil properties, soil organic matter (SOM) quantity and composition (the proportion of humic-like vs. protein-like OM), microbial biomass, enzyme activities and functional gene abundances in natural soils spanning an elevational gradient of ∼2400 m in Switzerland. Soil CO2 fluxes did not significantly vary from low (lowland zone) to higher (montane and subalpine zones) elevation forests, but decreased significantly (P<0.001) from the treeline to the mountain summit. Multivariate analyses revealed that CO2 fluxes were controlled by C-acquiring enzymatic activities which were mainly controlled by air mean annual temperature (MAT) and SOM quantity and composition. CH4 fluxes were characterized by uptake of atmospheric CH4, but no trend was observed along the elevation. N2O fluxes were also dominated by uptake of atmospheric N2O. The flux rates remained stable with increasing elevation below the treeline, but decreased significantly (P<0.001) from the treeline to the summit. N2O fluxes were driven by specific nitrifying and denitrifying microbial genes (ammonia-oxidizing amoA and N2O-producing norB), which were again controlled by SOM quantity and composition. Our study indicates the treeline as a demarcation point changing the patterns of CO2 and N2O fluxes along the elevation, highlighting the importance of SOM quantity and composition in controlling microbial enzyme activities and GHG fluxes.

Original languageEnglish
Article number116993
JournalGeoderma
Volume449
Number of pages14
ISSN0016-7061
DOIs
Publication statusPublished - 2024

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Keywords

  • Elevational gradient
  • Greenhouse gas fluxes
  • Microbial enzyme activities and gene functions
  • Mountainous soils
  • SOM quantity and composition

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