Structured illumination in Fresnel biprism-based digital holographic microscopy

S. Hossein S. Yaghoubi; Samira Ebrahimi; Masoomeh Dashtdar

doi:10.1016/j.optlaseng.2022.107215

Structured illumination in Fresnel biprism-based digital holographic microscopy

S. Hossein S. Yaghoubi, Samira Ebrahimi, Masoomeh Dashtdar^*

^*Corresponding author for this work

Section for Plant Glycobiology

Research output: Contribution to journal › Journal article › Research › peer-review

7 Citations (Scopus)

11 Downloads (Pure)

Abstract

The structured illumination (SI) architecture can be adopted in a digital holographic (DH) microscope to enhance the spatial resolution. In this paper, we propose and demonstrate a compact and simple SI method in a self-referencing common-path configuration of DH microscopy. The combination of SI pattern generated by a compact disk and common-path off-axis geometry formed by Fresnel biprism introduces a low-cost and highly stable system to image both amplitude and phase objects with a twofold improvement of the spatial resolution. Experimental results for the case of a standard test sample validate the predicted resolution enhancement compared to that of the conventional imager. The presented DH method allows for imaging living cells with more detail compared to the conventional DH microscopes.

Original language	English
Article number	107215
Journal	Optics and Lasers in Engineering
Volume	159
ISSN	0143-8166
DOIs	https://doi.org/10.1016/j.optlaseng.2022.107215
Publication status	Published - 2022

Bibliographical note

Publisher Copyright:
© 2022

Keywords

Biomedical imaging
common-path digital holographic microscopy
Fresnel Biprism
resolution enhancement
Structured illumination

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10.1016/j.optlaseng.2022.107215

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Cite this

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title = "Structured illumination in Fresnel biprism-based digital holographic microscopy",

abstract = "The structured illumination (SI) architecture can be adopted in a digital holographic (DH) microscope to enhance the spatial resolution. In this paper, we propose and demonstrate a compact and simple SI method in a self-referencing common-path configuration of DH microscopy. The combination of SI pattern generated by a compact disk and common-path off-axis geometry formed by Fresnel biprism introduces a low-cost and highly stable system to image both amplitude and phase objects with a twofold improvement of the spatial resolution. Experimental results for the case of a standard test sample validate the predicted resolution enhancement compared to that of the conventional imager. The presented DH method allows for imaging living cells with more detail compared to the conventional DH microscopes.",

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T1 - Structured illumination in Fresnel biprism-based digital holographic microscopy

AU - Yaghoubi, S. Hossein S.

AU - Ebrahimi, Samira

AU - Dashtdar, Masoomeh

PY - 2022

Y1 - 2022

N2 - The structured illumination (SI) architecture can be adopted in a digital holographic (DH) microscope to enhance the spatial resolution. In this paper, we propose and demonstrate a compact and simple SI method in a self-referencing common-path configuration of DH microscopy. The combination of SI pattern generated by a compact disk and common-path off-axis geometry formed by Fresnel biprism introduces a low-cost and highly stable system to image both amplitude and phase objects with a twofold improvement of the spatial resolution. Experimental results for the case of a standard test sample validate the predicted resolution enhancement compared to that of the conventional imager. The presented DH method allows for imaging living cells with more detail compared to the conventional DH microscopes.

AB - The structured illumination (SI) architecture can be adopted in a digital holographic (DH) microscope to enhance the spatial resolution. In this paper, we propose and demonstrate a compact and simple SI method in a self-referencing common-path configuration of DH microscopy. The combination of SI pattern generated by a compact disk and common-path off-axis geometry formed by Fresnel biprism introduces a low-cost and highly stable system to image both amplitude and phase objects with a twofold improvement of the spatial resolution. Experimental results for the case of a standard test sample validate the predicted resolution enhancement compared to that of the conventional imager. The presented DH method allows for imaging living cells with more detail compared to the conventional DH microscopes.

KW - Biomedical imaging

KW - common-path digital holographic microscopy

KW - Fresnel Biprism

KW - resolution enhancement

KW - Structured illumination

U2 - 10.1016/j.optlaseng.2022.107215

DO - 10.1016/j.optlaseng.2022.107215

M3 - Journal article

AN - SCOPUS:85136092088

VL - 159

JO - Optics and Lasers in Engineering

JF - Optics and Lasers in Engineering

SN - 0143-8166

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