Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorum-sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model

Louise Christensen, Maria van Gennip, Tim H Jakobsen, Morten Alhede, Hans Petter Hougen, Niels Høiby, Thomas Bjarnsholt, Michael Givskov, Maria Alhede

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151 Citations (Scopus)

Abstract

Quorum sensing (QS)-deficient Pseudomonas aeruginosa biofilms formed in vitro are more susceptible to tobramycin than QS-proficient P. aeruginosa biofilms, and combination treatment with a QS inhibitor (QSI) and tobramycin shows synergistic effects on the killing of in vitro biofilms. We extended these results to an in vivo P. aeruginosa foreign-body biofilm model. The effect of treatment initiated prophylactically was compared with treatment initiated 11 days post-insertion.
Original languageEnglish
JournalThe Journal of antimicrobial chemotherapy
Volume67
Issue number5
Pages (from-to)1198-206
Number of pages9
ISSN0305-7453
DOIs
Publication statusPublished - May 2012

Keywords

  • Animals
  • Anti-Bacterial Agents
  • Bacterial Load
  • Disease Models, Animal
  • Drug Synergism
  • Drug Therapy, Combination
  • Female
  • Foreign Bodies
  • Histocytochemistry
  • Mice
  • Mice, Inbred BALB C
  • Peritonitis
  • Pseudomonas Infections
  • Pseudomonas aeruginosa
  • Quorum Sensing
  • Tobramycin

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