Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorum-sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model

Louise Christensen; Maria van Gennip; Tim H Jakobsen; Morten Alhede; Hans Petter Hougen; Niels Høiby; Thomas Bjarnsholt; Michael Givskov; Maria Alhede

doi:10.1093/jac/dks002

Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorum-sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model

Louise Christensen, Maria van Gennip, Tim H Jakobsen, Morten Alhede, Hans Petter Hougen, Niels Høiby, Thomas Bjarnsholt, Michael Givskov, Maria Alhede

Section of Forensic Pathology

Research output: Contribution to journal › Journal article › Research › peer-review

153 Citations (Scopus)

Abstract

Quorum sensing (QS)-deficient Pseudomonas aeruginosa biofilms formed in vitro are more susceptible to tobramycin than QS-proficient P. aeruginosa biofilms, and combination treatment with a QS inhibitor (QSI) and tobramycin shows synergistic effects on the killing of in vitro biofilms. We extended these results to an in vivo P. aeruginosa foreign-body biofilm model. The effect of treatment initiated prophylactically was compared with treatment initiated 11 days post-insertion.

Original language	English
Journal	The Journal of antimicrobial chemotherapy
Volume	67
Issue number	5
Pages (from-to)	1198-206
Number of pages	9
ISSN	0305-7453
DOIs	https://doi.org/10.1093/jac/dks002
Publication status	Published - May 2012

Keywords

Animals
Anti-Bacterial Agents
Bacterial Load
Disease Models, Animal
Drug Synergism
Drug Therapy, Combination
Female
Foreign Bodies
Histocytochemistry
Mice
Mice, Inbred BALB C
Peritonitis
Pseudomonas Infections
Pseudomonas aeruginosa
Quorum Sensing
Tobramycin

Access to Document

10.1093/jac/dks002

Cite this

Christensen, L., van Gennip, M., Jakobsen, T. H., Alhede, M., Hougen, H. P., Høiby, N., Bjarnsholt, T., Givskov, M., & Alhede, M. (2012). Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorum-sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model. The Journal of antimicrobial chemotherapy, 67(5), 1198-206. https://doi.org/10.1093/jac/dks002

Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorum-sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model. / Christensen, Louise; van Gennip, Maria; Jakobsen, Tim H; Alhede, Morten; Hougen, Hans Petter; Høiby, Niels ; Bjarnsholt, Thomas ; Givskov, Michael; Alhede, Maria.

In: The Journal of antimicrobial chemotherapy, Vol. 67, No. 5, 05.2012, p. 1198-206.

Research output: Contribution to journal › Journal article › Research › peer-review

Christensen, L, van Gennip, M, Jakobsen, TH, Alhede, M, Hougen, HP, Høiby, N , Bjarnsholt, T , Givskov, M & Alhede, M 2012, 'Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorum-sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model', The Journal of antimicrobial chemotherapy, vol. 67, no. 5, pp. 1198-206. https://doi.org/10.1093/jac/dks002

Christensen L, van Gennip M, Jakobsen TH, Alhede M, Hougen HP, Høiby N et al. Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorum-sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model. The Journal of antimicrobial chemotherapy. 2012 May;67(5):1198-206. https://doi.org/10.1093/jac/dks002

Christensen, Louise ; van Gennip, Maria ; Jakobsen, Tim H ; Alhede, Morten ; Hougen, Hans Petter ; Høiby, Niels ; Bjarnsholt, Thomas ; Givskov, Michael ; Alhede, Maria. / Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorum-sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model. In: The Journal of antimicrobial chemotherapy. 2012 ; Vol. 67, No. 5. pp. 1198-206.

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