Tandem malonate-based glucosides (TMGs) for membrane protein structural studies

Hazrat Hussain, Jonas S. Mortensen, Yang Du, Claudia Santillan, Orquidea Ribeiro, Juyeon Go, Parameswaran Hariharan, Claus J. Loland, Lan Guan, Brian K. Kobilka, Bernadette Byrne, Pil Seok Chae*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

11 Citations (Scopus)
182 Downloads (Pure)

Abstract

High-resolution membrane protein structures are essential for understanding the molecular basis of diverse biological events and important in drug development. Detergents are usually used to extract these bio-macromolecules from the membranes and maintain them in a soluble and stable state in aqueous solutions for downstream characterization. However, many eukaryotic membrane proteins solubilized in conventional detergents tend to undergo structural degradation, necessitating the development of new amphiphilic agents with enhanced properties. In this study, we designed and synthesized a novel class of glucoside amphiphiles, designated tandem malonate-based glucosides (TMGs). A few TMG agents proved effective at both stabilizing a range of membrane proteins and extracting proteins from the membrane environment. These favourable characteristics, along with synthetic convenience, indicate that these agents have potential in membrane protein research.

Original languageEnglish
Article number3963
JournalScientific Reports
Volume7
Issue number1
Number of pages11
ISSN2045-2322
DOIs
Publication statusPublished - 2017

Cite this