The effect of pH and ionic strength on the fluorescence properties of a red emissive DNA-stabilized silver nanocluster

Marta Gambucci; Cecilia Cerretani; Loredana Latterini; Tom Vosch

doi:10.1088/2050-6120/ab47f2

The effect of pH and ionic strength on the fluorescence properties of a red emissive DNA-stabilized silver nanocluster

Marta Gambucci, Cecilia Cerretani, Loredana Latterini, Tom Vosch

Department of Chemistry

Research output: Contribution to journal › Journal article › Research › peer-review

16 Citations (Scopus)

Abstract

DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of promising fluorophores for imaging and sensing applications. All aspects of their spectroscopic properties are not yet fully characterized, leaving this field still with a number of fundamental studies to be addressed. In this work, we studied the spectroscopic properties of red-emitting DNA-AgNCs at different pH (5 to 9) and ionic strength μ (0.005 to 0.5). The photophysical properties of high performance liquid chromatography (HPLC) purified DNA-AgNCs proved to be constant over a large range of pH and μ, with absorption, emission and fluorescence decay times only being affected at very high pH and μ values. Non-purified DNA-AgNCs were also unaffected by pH and/or μ variations, but significant differences can be observed between the rotational correlation times of purified and non-purified DNA-AgNCs.

Original language	English
Journal	Methods and Applications in Fluorescence
Volume	8
Issue number	1
Pages (from-to)	014005
Number of pages	8
ISSN	2050-6120
DOIs	https://doi.org/10.1088/2050-6120/ab47f2
Publication status	Published - 3 Dec 2019

Keywords

Biosensing Techniques
Chromatography, High Pressure Liquid
DNA/chemistry
Fluorescence
Hydrogen-Ion Concentration
Metal Nanoparticles/chemistry
Osmolar Concentration
Silver/chemistry
Spectrometry, Fluorescence
Time Factors

Access to Document

10.1088/2050-6120/ab47f2

Cite this

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title = "The effect of pH and ionic strength on the fluorescence properties of a red emissive DNA-stabilized silver nanocluster",

abstract = "DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of promising fluorophores for imaging and sensing applications. All aspects of their spectroscopic properties are not yet fully characterized, leaving this field still with a number of fundamental studies to be addressed. In this work, we studied the spectroscopic properties of red-emitting DNA-AgNCs at different pH (5 to 9) and ionic strength μ (0.005 to 0.5). The photophysical properties of high performance liquid chromatography (HPLC) purified DNA-AgNCs proved to be constant over a large range of pH and μ, with absorption, emission and fluorescence decay times only being affected at very high pH and μ values. Non-purified DNA-AgNCs were also unaffected by pH and/or μ variations, but significant differences can be observed between the rotational correlation times of purified and non-purified DNA-AgNCs.",

keywords = "Biosensing Techniques, Chromatography, High Pressure Liquid, DNA/chemistry, Fluorescence, Hydrogen-Ion Concentration, Metal Nanoparticles/chemistry, Osmolar Concentration, Silver/chemistry, Spectrometry, Fluorescence, Time Factors",

author = "Marta Gambucci and Cecilia Cerretani and Loredana Latterini and Tom Vosch",

year = "2019",

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doi = "10.1088/2050-6120/ab47f2",

language = "English",

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T1 - The effect of pH and ionic strength on the fluorescence properties of a red emissive DNA-stabilized silver nanocluster

AU - Gambucci, Marta

AU - Cerretani, Cecilia

AU - Latterini, Loredana

AU - Vosch, Tom

PY - 2019/12/3

Y1 - 2019/12/3

N2 - DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of promising fluorophores for imaging and sensing applications. All aspects of their spectroscopic properties are not yet fully characterized, leaving this field still with a number of fundamental studies to be addressed. In this work, we studied the spectroscopic properties of red-emitting DNA-AgNCs at different pH (5 to 9) and ionic strength μ (0.005 to 0.5). The photophysical properties of high performance liquid chromatography (HPLC) purified DNA-AgNCs proved to be constant over a large range of pH and μ, with absorption, emission and fluorescence decay times only being affected at very high pH and μ values. Non-purified DNA-AgNCs were also unaffected by pH and/or μ variations, but significant differences can be observed between the rotational correlation times of purified and non-purified DNA-AgNCs.

AB - DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of promising fluorophores for imaging and sensing applications. All aspects of their spectroscopic properties are not yet fully characterized, leaving this field still with a number of fundamental studies to be addressed. In this work, we studied the spectroscopic properties of red-emitting DNA-AgNCs at different pH (5 to 9) and ionic strength μ (0.005 to 0.5). The photophysical properties of high performance liquid chromatography (HPLC) purified DNA-AgNCs proved to be constant over a large range of pH and μ, with absorption, emission and fluorescence decay times only being affected at very high pH and μ values. Non-purified DNA-AgNCs were also unaffected by pH and/or μ variations, but significant differences can be observed between the rotational correlation times of purified and non-purified DNA-AgNCs.

KW - Biosensing Techniques

KW - Chromatography, High Pressure Liquid

KW - DNA/chemistry

KW - Fluorescence

KW - Hydrogen-Ion Concentration

KW - Metal Nanoparticles/chemistry

KW - Osmolar Concentration

KW - Silver/chemistry

KW - Spectrometry, Fluorescence

KW - Time Factors

U2 - 10.1088/2050-6120/ab47f2

DO - 10.1088/2050-6120/ab47f2

M3 - Journal article

C2 - 31794430

VL - 8

SP - 014005

JO - Methods and Applications in Fluorescence

JF - Methods and Applications in Fluorescence

SN - 2050-6120

IS - 1

ER -