The rapid “teabag” method for high-end purification of membrane proteins

Jenny Hering, Julie Winkel Missel, Liying Zhang, Anders Gunnarsson, Marie Castaldo, Per Amstrup Pedersen, Margareta Ek, Pontus Gourdon, Harm Jan Snijder*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Overproduction and purification of membrane proteins are generally challenging and time-consuming procedures due to low expression levels, misfolding, and low stability once extracted from the membrane. Reducing processing steps and shortening the timespan for purification represent attractive approaches to overcome some of these challenges. We have therefore compared a fast “teabag” purification method with conventional purification for five different membrane proteins (MraY, AQP10, ClC-1, PAR2 and KCC2). Notably, this new approach reduces the purification time significantly, and the quality of the purified membrane proteins is equal to or exceeds conventional methods as assessed by size exclusion chromatography, SDS-PAGE and downstream applications such as ITC, crystallization and cryo-EM. Furthermore, the method is scalable, applicable to a range of affinity resins and allows for parallelization. Consequently, the technique has the potential to substantially simplify purification efforts of membrane proteins in basic and applied sciences.

Original languageEnglish
Article number16167
JournalScientific Reports
Volume10
Number of pages11
ISSN2045-2322
DOIs
Publication statusPublished - 2020

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