Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses

Alexander Jönsson; Rasmus R Svejdal; Nanna Bøgelund; Tam T T N Nguyen; Henrik Flindt; Jörg P Kutter; Kasper D Rand; Josiane P Lafleur

doi:10.1021/acs.analchem.6b05103

Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses

Alexander Jönsson, Rasmus R Svejdal, Nanna Bøgelund, Tam T T N Nguyen, Henrik Flindt, Jörg P Kutter, Kasper D Rand, Josiane P Lafleur

Analytical Biosciences

Research output: Contribution to journal › Journal article › Research › peer-review

42 Citations (Scopus)

Abstract

To improve the sample handling, and reduce cost and preparation time, of peptide mapping LC-MS workflows in protein analytical research, we here investigate the possibility of replacing conventional enzymatic digestion methods with a polymer microfluidic chip based enzyme reactor. Off-stoichiometric thiol-ene is utilized as both bulk material and as a monolithic stationary phase for immobilization of the proteolytic enzyme pepsin. The digestion efficiency of the, thiol-ene based, immobilized enzyme reactor (IMER) is compared to that of a conventional, agarose packed bed, pepsin IMER column commonly used in LC-MS based protein analyses. The chip IMER is found to rival the conventional column in terms of digestion efficiency at comparable residence time and, using a 3D-printed interface, be directly interfaceable with LC-MS.

Original language	English
Journal	Analytical Chemistry
Volume	89
Issue number	8
Pages (from-to)	4573-4580
Number of pages	8
ISSN	0003-2700
DOIs	https://doi.org/10.1021/acs.analchem.6b05103
Publication status	Published - 21 Mar 2017

Keywords

Journal Article

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Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses. / Jönsson, Alexander; Svejdal, Rasmus R; Bøgelund, Nanna; Nguyen, Tam T T N; Flindt, Henrik; Kutter, Jörg P ; Rand, Kasper D; Lafleur, Josiane P.

In: Analytical Chemistry, Vol. 89, No. 8, 21.03.2017, p. 4573-4580.

Research output: Contribution to journal › Journal article › Research › peer-review

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AU - Nguyen, Tam T T N

AU - Flindt, Henrik

AU - Kutter, Jörg P

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AU - Lafleur, Josiane P

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