Towards the development of Self-Nano-Emulsifying Drug Delivery Systems (SNEDDS) containing trimethyl chitosan for the oral delivery of amphotericin B: In vitro assessment and cytocompatibility studies

Eleni Kontogiannidou; Thomas Meikopoulos; Christina Virgiliou; Nikolaos Bouropoulos; Helen Gika; Ioannis S. Vizirianakis; Anette Mullertz; Dimitrios G. Fatouros

doi:10.1016/j.jddst.2020.101524

Towards the development of Self-Nano-Emulsifying Drug Delivery Systems (SNEDDS) containing trimethyl chitosan for the oral delivery of amphotericin B: In vitro assessment and cytocompatibility studies

Eleni Kontogiannidou, Thomas Meikopoulos, Christina Virgiliou, Nikolaos Bouropoulos, Helen Gika, Ioannis S. Vizirianakis, Anette Mullertz, Dimitrios G. Fatouros^*

^*Corresponding author for this work

Physiological Pharmaceutics

Research output: Contribution to journal › Journal article › Research › peer-review

24 Citations (Scopus)

Abstract

In the current study, a self-nanoemulsifying drug delivery system (SNEDDS) containing N-trimethyl chitosan chloride (TMC) was developed and evaluated for its potential to deliver amphotericin B (AmpB) via the gastrointestinal tract. SNEDDS comprising Captex 355, Kolliphor RH40 and Propylene Glycol were optimized and characterized for their formation spontaneity, droplet size and drug loading. Investigations on the effects of the application SNEDDS containing TMC to a model intestinal epithelium (Caco-2 monolayer) indicated the capability of the formulations to induce the transient opening of tight junctions. Caco-2 cell viability studies confirmed the safety of the SNEDDS, whereas in vitro transport studies of AmpB through Caco-2 cell monolayers showed the permeation enhancing ability of TMC. Our results suggest that chitosan derivative TMC combined with a SNEDDS may be used as permeation enhancer to facilitate oral delivery of AmpB.

Original language	English
Article number	101524
Journal	Journal of Drug Delivery Science and Technology
Volume	56
Number of pages	9
ISSN	1773-2247
DOIs	https://doi.org/10.1016/j.jddst.2020.101524
Publication status	Published - Apr 2020

Keywords

Trimethyl chitosan
Self-emulsifying drug delivery systems
Amphotericin B
Oral drug delivery
Caco-2 cells
MUCOADHESIVE PROPERTIES
VISCERAL LEISHMANIASIS
INTESTINAL-ABSORPTION
FORMULATION
QUATERNIZATION
NANOPARTICLES
TRANSPORT
CHLORIDE
INSULIN
BIODISTRIBUTION

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10.1016/j.jddst.2020.101524

Cite this

Kontogiannidou, E., Meikopoulos, T., Virgiliou, C., Bouropoulos, N., Gika, H., Vizirianakis, I. S., Mullertz, A., & Fatouros, D. G. (2020). Towards the development of Self-Nano-Emulsifying Drug Delivery Systems (SNEDDS) containing trimethyl chitosan for the oral delivery of amphotericin B: In vitro assessment and cytocompatibility studies. Journal of Drug Delivery Science and Technology, 56, [101524]. https://doi.org/10.1016/j.jddst.2020.101524

Towards the development of Self-Nano-Emulsifying Drug Delivery Systems (SNEDDS) containing trimethyl chitosan for the oral delivery of amphotericin B : In vitro assessment and cytocompatibility studies. / Kontogiannidou, Eleni; Meikopoulos, Thomas; Virgiliou, Christina; Bouropoulos, Nikolaos; Gika, Helen; Vizirianakis, Ioannis S.; Mullertz, Anette; Fatouros, Dimitrios G.

In: Journal of Drug Delivery Science and Technology, Vol. 56, 101524, 04.2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Kontogiannidou, E, Meikopoulos, T, Virgiliou, C, Bouropoulos, N, Gika, H, Vizirianakis, IS, Mullertz, A & Fatouros, DG 2020, 'Towards the development of Self-Nano-Emulsifying Drug Delivery Systems (SNEDDS) containing trimethyl chitosan for the oral delivery of amphotericin B: In vitro assessment and cytocompatibility studies', Journal of Drug Delivery Science and Technology, vol. 56, 101524. https://doi.org/10.1016/j.jddst.2020.101524

Kontogiannidou E, Meikopoulos T, Virgiliou C, Bouropoulos N, Gika H, Vizirianakis IS et al. Towards the development of Self-Nano-Emulsifying Drug Delivery Systems (SNEDDS) containing trimethyl chitosan for the oral delivery of amphotericin B: In vitro assessment and cytocompatibility studies. Journal of Drug Delivery Science and Technology. 2020 Apr;56. 101524. https://doi.org/10.1016/j.jddst.2020.101524

Kontogiannidou, Eleni ; Meikopoulos, Thomas ; Virgiliou, Christina ; Bouropoulos, Nikolaos ; Gika, Helen ; Vizirianakis, Ioannis S. ; Mullertz, Anette ; Fatouros, Dimitrios G. / Towards the development of Self-Nano-Emulsifying Drug Delivery Systems (SNEDDS) containing trimethyl chitosan for the oral delivery of amphotericin B : In vitro assessment and cytocompatibility studies. In: Journal of Drug Delivery Science and Technology. 2020 ; Vol. 56.

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abstract = "In the current study, a self-nanoemulsifying drug delivery system (SNEDDS) containing N-trimethyl chitosan chloride (TMC) was developed and evaluated for its potential to deliver amphotericin B (AmpB) via the gastrointestinal tract. SNEDDS comprising Captex 355, Kolliphor RH40 and Propylene Glycol were optimized and characterized for their formation spontaneity, droplet size and drug loading. Investigations on the effects of the application SNEDDS containing TMC to a model intestinal epithelium (Caco-2 monolayer) indicated the capability of the formulations to induce the transient opening of tight junctions. Caco-2 cell viability studies confirmed the safety of the SNEDDS, whereas in vitro transport studies of AmpB through Caco-2 cell monolayers showed the permeation enhancing ability of TMC. Our results suggest that chitosan derivative TMC combined with a SNEDDS may be used as permeation enhancer to facilitate oral delivery of AmpB.",

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author = "Eleni Kontogiannidou and Thomas Meikopoulos and Christina Virgiliou and Nikolaos Bouropoulos and Helen Gika and Vizirianakis, {Ioannis S.} and Anette Mullertz and Fatouros, {Dimitrios G.}",

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AU - Meikopoulos, Thomas

AU - Virgiliou, Christina

AU - Bouropoulos, Nikolaos

AU - Gika, Helen

AU - Vizirianakis, Ioannis S.

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AU - Fatouros, Dimitrios G.

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N2 - In the current study, a self-nanoemulsifying drug delivery system (SNEDDS) containing N-trimethyl chitosan chloride (TMC) was developed and evaluated for its potential to deliver amphotericin B (AmpB) via the gastrointestinal tract. SNEDDS comprising Captex 355, Kolliphor RH40 and Propylene Glycol were optimized and characterized for their formation spontaneity, droplet size and drug loading. Investigations on the effects of the application SNEDDS containing TMC to a model intestinal epithelium (Caco-2 monolayer) indicated the capability of the formulations to induce the transient opening of tight junctions. Caco-2 cell viability studies confirmed the safety of the SNEDDS, whereas in vitro transport studies of AmpB through Caco-2 cell monolayers showed the permeation enhancing ability of TMC. Our results suggest that chitosan derivative TMC combined with a SNEDDS may be used as permeation enhancer to facilitate oral delivery of AmpB.

AB - In the current study, a self-nanoemulsifying drug delivery system (SNEDDS) containing N-trimethyl chitosan chloride (TMC) was developed and evaluated for its potential to deliver amphotericin B (AmpB) via the gastrointestinal tract. SNEDDS comprising Captex 355, Kolliphor RH40 and Propylene Glycol were optimized and characterized for their formation spontaneity, droplet size and drug loading. Investigations on the effects of the application SNEDDS containing TMC to a model intestinal epithelium (Caco-2 monolayer) indicated the capability of the formulations to induce the transient opening of tight junctions. Caco-2 cell viability studies confirmed the safety of the SNEDDS, whereas in vitro transport studies of AmpB through Caco-2 cell monolayers showed the permeation enhancing ability of TMC. Our results suggest that chitosan derivative TMC combined with a SNEDDS may be used as permeation enhancer to facilitate oral delivery of AmpB.

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KW - BIODISTRIBUTION

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