Transport engineering in microbial cell factories producing plant-specialized metabolites

Zeinu Mussa Belew; Michal Poborsky; Hussam Hassan Nour-Eldin; Barbara Ann Halkier

doi:10.1016/j.cogsc.2021.100576

Transport engineering in microbial cell factories producing plant-specialized metabolites

Zeinu Mussa Belew, Michal Poborsky, Hussam Hassan Nour-Eldin, Barbara Ann Halkier^*

^*Corresponding author for this work

Section for Molecular Plant Biology

Research output: Contribution to journal › Review › Research › peer-review

8 Citations (Scopus)

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Abstract

Transport engineering strategies use altered expression of transporter proteins to change metabolite distribution within an organism. The production of plant specialized metabolites in microbial cell factories encounters a set of challenges that could benefit from the implementation of transport engineering technology. The range of challenges includes premature pathway termination due to secretion of intermediates, feedback inhibition due to inefficient export of final products, low yields in bioconversion processes due to inefficient import of precursors, and poor connectivity between subcellular compartments expressing different parts of complex biosynthetic pathways. We highlight the latest examples of transport engineering in microbial cell factories producing plant specialized metabolites, identify the current knowledge gap, and propose future research for advancing the field.

Original language	English
Article number	100576
Journal	Current Opinion in Green and Sustainable Chemistry
Volume	33
ISSN	2452-2236
DOIs	https://doi.org/10.1016/j.cogsc.2021.100576
Publication status	Published - 2022

Bibliographical note

Publisher Copyright:
© 2021 Elsevier B.V.

Keywords

Feedback inhibition
Metabolic engineering
Microbial cell factories
Pathway engineering
Plant specialized metabolites
Transport engineering

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10.1016/j.cogsc.2021.100576Licence: CC BY

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Cite this

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title = "Transport engineering in microbial cell factories producing plant-specialized metabolites",

abstract = "Transport engineering strategies use altered expression of transporter proteins to change metabolite distribution within an organism. The production of plant specialized metabolites in microbial cell factories encounters a set of challenges that could benefit from the implementation of transport engineering technology. The range of challenges includes premature pathway termination due to secretion of intermediates, feedback inhibition due to inefficient export of final products, low yields in bioconversion processes due to inefficient import of precursors, and poor connectivity between subcellular compartments expressing different parts of complex biosynthetic pathways. We highlight the latest examples of transport engineering in microbial cell factories producing plant specialized metabolites, identify the current knowledge gap, and propose future research for advancing the field.",

keywords = "Feedback inhibition, Metabolic engineering, Microbial cell factories, Pathway engineering, Plant specialized metabolites, Transport engineering",

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journal = "Current Opinion in Green and Sustainable Chemistry",

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T1 - Transport engineering in microbial cell factories producing plant-specialized metabolites

AU - Belew, Zeinu Mussa

AU - Poborsky, Michal

AU - Nour-Eldin, Hussam Hassan

AU - Halkier, Barbara Ann

PY - 2022

Y1 - 2022

N2 - Transport engineering strategies use altered expression of transporter proteins to change metabolite distribution within an organism. The production of plant specialized metabolites in microbial cell factories encounters a set of challenges that could benefit from the implementation of transport engineering technology. The range of challenges includes premature pathway termination due to secretion of intermediates, feedback inhibition due to inefficient export of final products, low yields in bioconversion processes due to inefficient import of precursors, and poor connectivity between subcellular compartments expressing different parts of complex biosynthetic pathways. We highlight the latest examples of transport engineering in microbial cell factories producing plant specialized metabolites, identify the current knowledge gap, and propose future research for advancing the field.

AB - Transport engineering strategies use altered expression of transporter proteins to change metabolite distribution within an organism. The production of plant specialized metabolites in microbial cell factories encounters a set of challenges that could benefit from the implementation of transport engineering technology. The range of challenges includes premature pathway termination due to secretion of intermediates, feedback inhibition due to inefficient export of final products, low yields in bioconversion processes due to inefficient import of precursors, and poor connectivity between subcellular compartments expressing different parts of complex biosynthetic pathways. We highlight the latest examples of transport engineering in microbial cell factories producing plant specialized metabolites, identify the current knowledge gap, and propose future research for advancing the field.

KW - Feedback inhibition

KW - Metabolic engineering

KW - Microbial cell factories

KW - Pathway engineering

KW - Plant specialized metabolites

KW - Transport engineering

U2 - 10.1016/j.cogsc.2021.100576

DO - 10.1016/j.cogsc.2021.100576

M3 - Review

AN - SCOPUS:85122105771

VL - 33

JO - Current Opinion in Green and Sustainable Chemistry

JF - Current Opinion in Green and Sustainable Chemistry

SN - 2452-2236

M1 - 100576

ER -