Travel light: Essential packing for membrane proteins with an active lifestyle

Nicolai Tidemand Johansen; Frederik Grønbæk Tidemand; Martin Cramer Pedersen; Lise Arleth

doi:10.1016/j.biochi.2022.07.014

Travel light: Essential packing for membrane proteins with an active lifestyle

Nicolai Tidemand Johansen^*, Frederik Grønbæk Tidemand, Martin Cramer Pedersen, Lise Arleth

^*Corresponding author for this work

Research output: Contribution to journal › Review › Research › peer-review

7 Citations (Scopus)

23 Downloads (Pure)

Abstract

We review the considerable progress during the recent decade in the endeavours of designing, optimising, and utilising carrier particle systems for structural and functional studies of membrane proteins in near-native environments. New and improved systems are constantly emerging, novel studies push the perceived limits of a given carrier system, and specific carrier systems consolidate and entrench themselves as the system of choice for particular classes of target membrane protein systems. This review covers the most frequently used carrier systems for such studies and emphasises similarities and differences between these systems as well as current trends and future directions for the field. Particular interest is devoted to the biophysical properties and membrane mimicking ability of each system and the manner in which this may impact an embedded membrane protein and an eventual structural or functional study.

Original language	English
Journal	Biochimie
Volume	205
Pages (from-to)	3-26
Number of pages	24
ISSN	0300-9084
DOIs	https://doi.org/10.1016/j.biochi.2022.07.014
Publication status	Published - 2023

Bibliographical note

Publisher Copyright:
© 2022 The Authors

Keywords

Amphiphilic polymers
Carrier system
Membrane protein
Micelle
Nanodisc

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10.1016/j.biochi.2022.07.014Licence: CC BY

FulltextFinal published version, 4 MBLicence: CC BY

Cite this

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title = "Travel light: Essential packing for membrane proteins with an active lifestyle",

abstract = "We review the considerable progress during the recent decade in the endeavours of designing, optimising, and utilising carrier particle systems for structural and functional studies of membrane proteins in near-native environments. New and improved systems are constantly emerging, novel studies push the perceived limits of a given carrier system, and specific carrier systems consolidate and entrench themselves as the system of choice for particular classes of target membrane protein systems. This review covers the most frequently used carrier systems for such studies and emphasises similarities and differences between these systems as well as current trends and future directions for the field. Particular interest is devoted to the biophysical properties and membrane mimicking ability of each system and the manner in which this may impact an embedded membrane protein and an eventual structural or functional study.",

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author = "Johansen, {Nicolai Tidemand} and Tidemand, {Frederik Gr{\o}nb{\ae}k} and Pedersen, {Martin Cramer} and Lise Arleth",

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AU - Tidemand, Frederik Grønbæk

AU - Pedersen, Martin Cramer

AU - Arleth, Lise

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AB - We review the considerable progress during the recent decade in the endeavours of designing, optimising, and utilising carrier particle systems for structural and functional studies of membrane proteins in near-native environments. New and improved systems are constantly emerging, novel studies push the perceived limits of a given carrier system, and specific carrier systems consolidate and entrench themselves as the system of choice for particular classes of target membrane protein systems. This review covers the most frequently used carrier systems for such studies and emphasises similarities and differences between these systems as well as current trends and future directions for the field. Particular interest is devoted to the biophysical properties and membrane mimicking ability of each system and the manner in which this may impact an embedded membrane protein and an eventual structural or functional study.

KW - Amphiphilic polymers

KW - Carrier system

KW - Membrane protein

KW - Micelle

KW - Nanodisc

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DO - 10.1016/j.biochi.2022.07.014

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