True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis

Vibeke Børsholt Rudkjøbing, Trine R Thomsen, Morten Alhede, Kasper N Kragh, Per H Nielsen, Ulla Johansen, Michael Givskov, Niels Høiby, Thomas Bjarnsholt

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    Abstract

    Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens.
    Original languageEnglish
    JournalJournal of Clinical Microbiology
    Volume49
    Issue number12
    Pages (from-to)4352-5
    Number of pages4
    ISSN0095-1137
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Bacteria
    • Biodiversity
    • Bronchopneumonia
    • Chronic Disease
    • Cluster Analysis
    • Cystic Fibrosis
    • DNA, Bacterial
    • DNA, Ribosomal
    • Humans
    • Molecular Sequence Data
    • Phylogeny
    • RNA, Ribosomal, 16S
    • Sequence Analysis, DNA

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