Two distinct clones of methicillin-resistant Staphylococcus aureus (MRSA) with the same USA300 pulsed-field gel electrophoresis profile: a potential pitfall for identification of USA300 community-associated MRSA

Anders Rhod Larsen; Richard Goering; Marc Stegger; Jodi A Lindsay; Katherine A Gould; Jason Hinds; Marit Sørum; Henrik Westh; Kit Boye; Robert Skov

doi:10.1128/JCM.00934-09

Two distinct clones of methicillin-resistant Staphylococcus aureus (MRSA) with the same USA300 pulsed-field gel electrophoresis profile: a potential pitfall for identification of USA300 community-associated MRSA

Anders Rhod Larsen, Richard Goering, Marc Stegger, Jodi A Lindsay, Katherine A Gould, Jason Hinds, Marit Sørum, Henrik Westh, Kit Boye, Robert Skov

Department of Clinical Medicine

Research output: Contribution to journal › Journal article › Research › peer-review

41 Citations (Scopus)

Abstract

Analysis of methicillin-resistant Staphylococcus aureus (MRSA) characterized as USA300 by pulsed-field gel electrophoresis identified two distinct clones. One was similar to community-associated USA300 MRSA (ST8-IVa, t008, and Panton-Valentine leukocidin positive). The second (ST8-IVa, t024, and PVL negative) had different molecular characteristics and epidemiology, suggesting independent evolution. We recommend spa typing and/or PCR to discriminate between the two clones.

Original language	English
Journal	Journal of Clinical Microbiology
Volume	47
Issue number	11
Pages (from-to)	3765-8
Number of pages	3
ISSN	0095-1137
DOIs	https://doi.org/10.1128/JCM.00934-09
Publication status	Published - 2009

Access to Document

10.1128/JCM.00934-09

Library access?

Check availability at the Royal Danish Library

Cite this

Larsen, A. R., Goering, R., Stegger, M., Lindsay, J. A., Gould, K. A., Hinds, J., Sørum, M., Westh, H., Boye, K., & Skov, R. (2009). Two distinct clones of methicillin-resistant Staphylococcus aureus (MRSA) with the same USA300 pulsed-field gel electrophoresis profile: a potential pitfall for identification of USA300 community-associated MRSA. Journal of Clinical Microbiology, 47(11), 3765-8. https://doi.org/10.1128/JCM.00934-09

Two distinct clones of methicillin-resistant Staphylococcus aureus (MRSA) with the same USA300 pulsed-field gel electrophoresis profile: a potential pitfall for identification of USA300 community-associated MRSA. / Larsen, Anders Rhod; Goering, Richard; Stegger, Marc et al.
In: Journal of Clinical Microbiology, Vol. 47, No. 11, 2009, p. 3765-8.

Research output: Contribution to journal › Journal article › Research › peer-review

Larsen, AR, Goering, R, Stegger, M, Lindsay, JA, Gould, KA, Hinds, J, Sørum, M, Westh, H, Boye, K & Skov, R 2009, 'Two distinct clones of methicillin-resistant Staphylococcus aureus (MRSA) with the same USA300 pulsed-field gel electrophoresis profile: a potential pitfall for identification of USA300 community-associated MRSA', Journal of Clinical Microbiology, vol. 47, no. 11, pp. 3765-8. https://doi.org/10.1128/JCM.00934-09

@article{aa3e324d58584cd4a1b95e28d5996a93,

title = "Two distinct clones of methicillin-resistant Staphylococcus aureus (MRSA) with the same USA300 pulsed-field gel electrophoresis profile: a potential pitfall for identification of USA300 community-associated MRSA",

abstract = "Analysis of methicillin-resistant Staphylococcus aureus (MRSA) characterized as USA300 by pulsed-field gel electrophoresis identified two distinct clones. One was similar to community-associated USA300 MRSA (ST8-IVa, t008, and Panton-Valentine leukocidin positive). The second (ST8-IVa, t024, and PVL negative) had different molecular characteristics and epidemiology, suggesting independent evolution. We recommend spa typing and/or PCR to discriminate between the two clones.",

author = "Larsen, {Anders Rhod} and Richard Goering and Marc Stegger and Lindsay, {Jodi A} and Gould, {Katherine A} and Jason Hinds and Marit S{\o}rum and Henrik Westh and Kit Boye and Robert Skov",

year = "2009",

doi = "10.1128/JCM.00934-09",

language = "English",

volume = "47",

pages = "3765--8",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "11",

}

TY - JOUR

T1 - Two distinct clones of methicillin-resistant Staphylococcus aureus (MRSA) with the same USA300 pulsed-field gel electrophoresis profile: a potential pitfall for identification of USA300 community-associated MRSA

AU - Larsen, Anders Rhod

AU - Goering, Richard

AU - Stegger, Marc

AU - Lindsay, Jodi A

AU - Gould, Katherine A

AU - Hinds, Jason

AU - Sørum, Marit

AU - Westh, Henrik

AU - Boye, Kit

AU - Skov, Robert

PY - 2009

Y1 - 2009

N2 - Analysis of methicillin-resistant Staphylococcus aureus (MRSA) characterized as USA300 by pulsed-field gel electrophoresis identified two distinct clones. One was similar to community-associated USA300 MRSA (ST8-IVa, t008, and Panton-Valentine leukocidin positive). The second (ST8-IVa, t024, and PVL negative) had different molecular characteristics and epidemiology, suggesting independent evolution. We recommend spa typing and/or PCR to discriminate between the two clones.

AB - Analysis of methicillin-resistant Staphylococcus aureus (MRSA) characterized as USA300 by pulsed-field gel electrophoresis identified two distinct clones. One was similar to community-associated USA300 MRSA (ST8-IVa, t008, and Panton-Valentine leukocidin positive). The second (ST8-IVa, t024, and PVL negative) had different molecular characteristics and epidemiology, suggesting independent evolution. We recommend spa typing and/or PCR to discriminate between the two clones.

U2 - 10.1128/JCM.00934-09

DO - 10.1128/JCM.00934-09

M3 - Journal article

SN - 0095-1137

VL - 47

SP - 3765

EP - 3768

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 11

ER -